A NEW PROCEDURE FOR EFFICIENT RECOVERY OF DNA, RNA, AND PROTEINS FROM LISTERIA CELLS BY RAPID LYSIS WITH A RECOMBINANT BACTERIOPHAGE ENDOLYSIN

被引：39

作者：

LOESSNER, MJ

SCHNEIDER, A

SCHERER, S

机构：

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 03期

关键词：

D O I：

10.1128/AEM.61.3.1150-1152.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lyric enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2- to 5-ml) cultures. Moreover, this approach should be very useful in analytical detection and differentiation of Listeria strains when the release of native nucleic acids or proteins is required.

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页码：1150 / 1152

页数：3

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