CHIMERISM IN PCR PRODUCTS FROM A MULTIGENE FAMILY

被引：6

作者：

BHAVSAR, D

ZHENG, H

DRYSDALE, J

机构：

[1] Department of Biochemistry, Tufts Medical School, Boston, MA 02111

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 205卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.2756

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes a potential hazard in obtaining sequences of a multigene family by amplifying the mixed population by the polymerase chain reaction with primers from conserved sequences. We describe a product which appears to be a chimera formed during the PCR reaction from two different but related sequences. (C) 1994 Academic Press, Inc.

引用

页码：944 / 947

页数：4

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