INTERESTERIFICATION OF BUTTERFAT BY COMMERCIAL MICROBIAL LIPASES IN A COSURFACTANT FREE MICROEMULSION SYSTEM

被引:19
作者
SAFARI, M [1 ]
KERMASHA, S [1 ]
机构
[1] MCGILL UNIV,DEPT FOOD SCI & AGR CHEM,ST ANNE BELLEVUE H9X 3V9,PQ,CANADA
关键词
BUTTERFAT; COSURFACTANT-FREE MICROEMULSION SYSTEM; INTERESTERIFICATION; LIPASE;
D O I
10.1007/BF02542263
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Lipase catalyzed interesterification of butterfat was carried out in a cosurfactant-free microemulsion system containing mixtures of Span 60 and Tween 60 (ICI Specialty Chemicals Altemix Inc., Brantford, Ontario, Canada) as surfactants. Four commercial lipases were used-Lipozyme 10,000L (Novo Nordisk, Copenhagen, Denmark) and N, D and MPA (Amano Pharmaceutical Co. Ltd., Nagoya, Japan). Stereospecific analyses of fractionated selected high-molecular weight triacylglycerols were performed by enzymatic deacylation with commercial pancreatic lipase, random generation of rac-1,2-diacylglycerols by Grignard degradation, synthesis of rac-phosphatidylcholines and a stereospecific release of sn-1,2 diacylglycerols by phospho lipase A,. The results showed that the hydrolytic affinity of commercial lipases demonstrated an acyl group specificity toward lower-molecular weight fatty acids C4-C14:0. Stereospecific analyses of fatty acids of interesterified selected triacylglycerols of butterfat catalyzed by lipase N demonstrated a 46% increase in the proportion of C18:1 cis Delta 9 at the sn-2 position, whereas those catalyzed by lipases MAP, D and Lipozyme 10,000L were enriched with C16:0 at the same position by 21, 35 and 41%, respectively.
引用
收藏
页码:969 / 973
页数:5
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