LECTIN RECOGNITION OF HOST-LIKE SACCHARIDE MOTIFS IN STREPTOCOCCAL CELL-WALL POLYSACCHARIDES

被引:64
作者
CISAR, JO
SANDBERG, AL
ABEYGUNAWARDANA, C
REDDY, GP
BUSH, CA
机构
[1] UNIV MARYLAND,DEPT CHEM & BIOCHEM,BALTIMORE,MD 21228
[2] JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM,BALTIMORE,MD 21205
关键词
BACTERIAL ADHESINS; CELLULAR RECOGNITION; LECTINS; MICROBIAL COLONIZATION; STREPTOCOCCAL POLYSACCHARIDES;
D O I
10.1093/glycob/5.7.655
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Viridans streptococci that participate in the microbial colonization of teeth have cell wall polysaccharides composed of linear phosphodiester-linked hexa- or heptasaccharide repeating units, each containing a host-like disaccharide motif, either Gal beta 1-->3GalNAc or GalNAc beta 1-->3Gal. Whereas strains with GalNAc beta 1-->3Gal-containing polysaccharides co-aggregated with streptococci that possess GalNAc-sensitive lectins, strains with either host-like moth co-aggregated with Actinomyces spp, The latter interactions reflected the specificity of Actinomyces spp, lectins for common features of Gal beta 1-->3GalNAc and GalNAc beta 1--> 3Gal, Thus, alpha-linked glycosides of both disaccharides were much more potent inhibitors of co-aggregation than Gal or GalNAc Six non-bacterial lectins also reacted with the streptococcal polysaccharides, In general, precipitation of each lectin with each polysaccharide involved binding of Gal or GalNAc within the host-like moths, but not saccharides outside these regions, The lectins of Ricinus communis, Abrus precatorius, Codium fragile and Agaricus bisporus were most reactive with the Gal beta 1-->3GalNAc-containing polysaccharides, the Wisteria floribunda lectin with the GalNAc beta 1-->3Gal-containing polysaccharides and the Bauhinia purpurea lectin with polysaccharides containing either disaccharide, Thus, lectin recognition of the streptococcal cell wall polysaccharides involved either the common or specific sides of the Gal beta 1-->3GalNAc and GalNAc beta 1-->3Gal moths present within these molecules.
引用
收藏
页码:655 / 662
页数:8
相关论文
共 32 条
[1]   COMPLETE STRUCTURE OF THE POLYSACCHARIDE FROM STREPTOCOCCUS-SANGUIS J22 [J].
ABEYGUNAWARDANA, C ;
BUSH, CA ;
CISAR, JO .
BIOCHEMISTRY, 1990, 29 (01) :234-248
[2]   COMPLETE STRUCTURE OF THE CELL-SURFACE POLYSACCHARIDE OF STREPTOCOCCUS-ORALIS-C104 - A 600-MHZ NMR-STUDY [J].
ABEYGUNAWARDANA, C ;
BUSH, CA ;
CISAR, JO .
BIOCHEMISTRY, 1991, 30 (35) :8568-8577
[3]   COMPLETE STRUCTURE OF THE CELL-SURFACE POLYSACCHARIDE OF STREPTOCOCCUS-ORALIS ATCC-10557 - A RECEPTOR FOR LECTIN-MEDIATED INTERBACTERIAL ADHERENCE [J].
ABEYGUNAWARDANA, C ;
BUSH, CA ;
CISAR, JO .
BIOCHEMISTRY, 1991, 30 (26) :6528-6540
[4]   THE COMPLETE STRUCTURE OF THE CAPSULAR POLYSACCHARIDE FROM STREPTOCOCCUS-SANGUIS.34 [J].
ABEYGUNAWARDANA, C ;
BUSH, CA ;
TJOA, SS ;
FENNESSEY, PV ;
MCNEIL, MR .
CARBOHYDRATE RESEARCH, 1989, 191 (02) :279-293
[5]  
BAENZIGER JU, 1979, J BIOL CHEM, V254, P9795
[6]   BINDING OF ACTINOMYCES-NAESLUNDII TO GLYCOSPHINGOLIPIDS [J].
BRENNAN, MJ ;
JORALMON, RA ;
CISAR, JO ;
SANDBERG, AL .
INFECTION AND IMMUNITY, 1987, 55 (02) :487-489
[7]   SPECIFICITY OF COAGGREGATION REACTIONS BETWEEN HUMAN ORAL STREPTOCOCCI AND STRAINS OF ACTINOMYCES-VISCOSUS OR ACTINOMYCES-NAESLUNDII [J].
CISAR, JO ;
KOLENBRANDER, PE ;
MCINTIRE, FC .
INFECTION AND IMMUNITY, 1979, 24 (03) :742-752
[8]  
CISAR JO, 1986, MICROBIAL LECTINS AG, P183
[9]   CARBOHYDRATE-BINDING SITES OF THE MANNOSE-SPECIFIC FIMBRIAL LECTINS OF ENTEROBACTERIA [J].
FIRON, N ;
OFEK, I ;
SHARON, N .
INFECTION AND IMMUNITY, 1984, 43 (03) :1088-1090
[10]   FACTORS AFFECTING BINDING OF GALACTO LIGANDS TO ACTINOMYCES-VISCOSUS LECTIN [J].
HEEB, MJ ;
MARINI, AM ;
GABRIEL, O .
INFECTION AND IMMUNITY, 1985, 47 (01) :61-67