RECOMBINANT HUMAN BASIC FIBROBLAST GROWTH-FACTOR (RHBFGF) INDUCES SECRETION OF NERVE GROWTH-FACTOR (NGF) IN CULTURED RAT ASTROGLIAL CELLS

被引：28

作者：

FUKUMOTO, H

KAKIHANA, M

SUNO, M

机构：

[1] Biology Research Laboratories, Research and Development Division, Takeda Chemical Ind., Ltd., Osaka

来源：

NEUROSCIENCE LETTERS | 1991年 / 122卷 / 02期

关键词：

HUMAN BFGF; NGF; ASTROCYTE; NEUROTROPHIC EFFECT; CELL CULTURE; CENTRAL NERVOUS SYSTEM;

D O I：

10.1016/0304-3940(91)90863-O

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Recombinant human basic fibroblast growth factor (rhbFGF) was found to induce secretion of nerve growth factor (NGF) in cultured astrocytes from rat brain. The induction was concentration dependent; a maximum increase of 2-fold was observed at concentrations of 3-10 ng/ml or rhbFGF. When rhbFGF was added to the astrocytes, the induction occurred within 8 h and leveled off after 12 h. Antiserum against rhbFGF completely blocked the induction. Maximum induction occurred under serum-free culture conditions and in cultures with a high cell density. These results suggest that rhbFGF may exert a neurotrophic effect on the central nervous system through the induction of NGF secretion by astrocytes.

引用

页码：221 / 224

页数：4

共 20 条

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