ANALYSIS OF DNA RESTRICTION FRAGMENTS GREATER THAN 5.7 MB IN SIZE FROM THE CENTROMERIC REGION OF HUMAN-CHROMOSOMES

被引:2
作者
ARN, PH
LI, X
SMITH, C
HSU, M
SCHWARTZ, DC
JABS, ELW
机构
[1] JOHNS HOPKINS UNIV,DEPT PEDIAT,CTR MED GENET,CMSC 1004,600 N WOLFE ST,BALTIMORE,MD 21205
[2] CARNEGIE INST WASHINGTON,BALTIMORE,MD 21210
关键词
D O I
10.1007/BF00352332
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pulsed electrophoresis was used to study the organization of the human centromeric region. Genomic DNA was digested with rare-cutting enzymes. DNA fragments from 0.2 to greater than 5.7 Mb were separated by electrophoresis and hybridized with alphoid and simple DNA repeats. Rare-cutting enzymes (Mlu I, Nar I, Not I, Nru I, Sal I, Sfi I, Sst II) demonstrated fewer restriction sites at centromeric regions than elsewhere in the genome. The enzyme Not I had the fewest restriction sites at centromeric regions. As much as 70% of these sequences from the centromeric region are present in Not I DNA fragments greater than 5.7 and estimated to be as large as 10 Mb in size. Other repetitive sequences such as short interspersed repeated segments (SINEs), long interspersed repeated segments (LINEs), ribosomal DNA, and minisatellite DNA that are not enriched at the centromeric region, are not enriched in Not I fragments of greater than 5.7 Mb in size.
引用
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页码:249 / 254
页数:6
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