OXIDATIVE-DEGRADATION OF AN ANTITUMOR (1-3)-BETA-D-GLUCAN, GRIFOLAN

被引:32
作者
NONO, I [1 ]
OHNO, N [1 ]
MASUDA, A [1 ]
OIKAWA, S [1 ]
YADOMAE, T [1 ]
机构
[1] NIPPON BEET SUGAR MFG CO LTD,CHUO KU,TOKYO 104,JAPAN
来源
JOURNAL OF PHARMACOBIO-DYNAMICS | 1991年 / 14卷 / 01期
关键词
(1-3)-BETA-D-GLUCAN; GRIFOLA-FRONDOSA; ANTITUMOR ACTIVITY; OXIDATIVE DEGRADATION; GRIFOLAN; CURDLAN;
D O I
10.1248/bpb1978.14.9
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
A (1-3)-beta-D-glucan, grifolan (GRN), recovered from the peritoneal cavity after 1 d from i.p. injection contained a significant amount of anionic metabolite(s) having lower M(r) than the parent GRN. In parallel with this observation, GRN induced peritoneal exudate cells exhibiting a higher level of oxidative burst than the non-stimulated, resident peritoneal cells. Chemical oxidation of GRN by active oxygen species such as (a) ascorbic acid-CuSO4, (b) hydrogen peroxide, (c) hydrogen peroxide-CuSO4, or (d) hypochlorous acid also produced anionic as well as lower M(r) degradation products. Under these experimental conditions the structural changes were remarkable and in the order of a < b < c < d. The products formed under the conditions (a) and (b) retained significant antitumor activity but those of (c) and (d) lost the activity. However, oxidation product(s) of curdlan, an antitumor inactive (1-3)-beta-D-glucan having no branch, by by condition (d) induced significant antitumor activity. These results suggested that oxidative degradation of (1-3)-beta-D-glucans produced some temporary active metabolites and then gradually changed to the inactive form. However, these active metabolites contribute less than the parent glucan on the whole activation mechanisms of the host by (1-3)-beta-D-glucans.
引用
收藏
页码:9 / 19
页数:11
相关论文
共 28 条
[1]   CHANGE OF BIOLOGICAL-ACTIVITIES OF (1-]3)-BETA-D-GLUCAN FROM GRIFOLA-FRONDOSA UPON MOLECULAR-WEIGHT REDUCTION BY HEAT-TREATMENT [J].
ADACHI, Y ;
OHNO, N ;
OHSAWA, M ;
OIKAWA, S ;
YADOMAE, T .
CHEMICAL & PHARMACEUTICAL BULLETIN, 1990, 38 (02) :477-481
[2]  
ADACHI Y, 1989, CHEM PHARM BULL, V37, P1838
[3]  
ADACHI Y, 1990, CHEM PHARM BULL, V38, P988
[4]  
CIRELLI AF, 1989, CARBOHYD RES, V190, P329
[5]  
CZOP JK, 1985, J IMMUNOL, V134, P2588
[6]  
HAMURO J, 1980, IMMUNOLOGY, V39, P551
[7]  
JANUSZ MJ, 1989, J IMMUNOL, V142, P959
[8]  
Kashimura N, 1988, Tanpakushitsu Kakusan Koso, V33, P3116
[9]  
MORIKAWA K, 1985, CANCER RES, V45, P3482
[10]  
MORIKAWA K, 1985, CANCER RES, V45, P1496