CLONAL ANALYSIS OF ESCHERICHIA-COLI SEROTYPE-O6 STRAINS FROM URINARY-TRACT INFECTIONS

被引：17

作者：

ZINGLER, G

OTT, M

BLUM, G

FALKENHAGEN, U

NAUMANN, G

SOKOLOWSKAKOHLER, W

HACKER, J

机构：

[1] UNIV WURZBURG, INST GENET & MIKROBIOL, RONTGENRING 11, W-8700 WURZBURG, GERMANY

[2] UNIV ROSTOCK, INST MED MIKROBIOL, O-2500 ROSTOCK, GERMANY

[3] HUMBOLDT UNIV, INST MED MIKROBIOL, O-1040 BERLIN, GERMANY

来源：

MICROBIAL PATHOGENESIS | 1992年 / 12卷 / 04期

关键词：

ESCHERICHIA-COLI SEROTYPE-O6; URINARY TRACT INFECTION; VIRULENCE FACTORS; CLONAL ANALYSIS; MOLECULAR EPIDEMIOLOGY;

D O I：

10.1016/0882-4010(92)90048-S

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A total of 36 Escherichia coli urinary tract isolates (UTI) of serotype 06, with different combinations of capsule (K) and flagellin (H) antigens, were analysed according to the outer membrane pattern (OMP), serum resistance properties, mannose-resistant hemagglutination using various types of erythrocytes, and also for the genetic presence and the expression of P-fimbriae, S fimbriae/F1C fimbriae, Type 1 fimbriae, aerobactin and hemolysin. Twenty selected strains were further analysed by pulsed field gel electrophoresis (PFGE), elaborating genomic profiles by XbaI cleavage and subsequent Southern hybridization to virulence-associated DNA probes. It could be shown that 06 UTI isolates represent a highly heterogeneous group of strains according to the occurrence and combination of these traits. Relatedness on the genetic and the phenotypic level was found for some of the strains exhibiting the same O:K:H:F serotype. DNA long-range mapping further indicated some interesting features, according to the copy number and the genomic linkage of virulence genes. © 1992.

引用

页码：299 / 310

页数：12

共 55 条

[1] 6 WIDESPREAD BACTERIAL CLONES AMONG ESCHERICHIA-COLI K1 ISOLATES [J].