ISOLATION OF HUMAN RETINAL GENES - RECOVERIN CDNA AND GENE

被引:72
作者
MURAKAMI, A [1 ]
YAJIMA, T [1 ]
INANA, G [1 ]
机构
[1] UNIV MIAMI,SCH MED,BASCOM PALMER EYE INST,1638 NW 10TH AVE,MIAMI,FL 33136
关键词
D O I
10.1016/S0006-291X(05)81483-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A human retina cDNA library enriched for retina-specific clones was prepared by subtraction with a non-retina population of cDNA in combination with polymerase chain reaction (PCR) amplifications. A highly retina-specific cDNA clone (1190 bp) was obtained through this library encoding a 200 amino acid protein with three calcium binding sites and 87% homology to the bovine photoreceptor protein, recoverin, which has been shown to mediate the recovery of the dark current after photoactivation, and 58% homology to the calcium-binding chick cone protein, visinin. Analysis of the gene indicated a 9-10 kb single-copy gene with at least three exons and two introns. The three exons contained the entire coding sequence, and all of the calcium-binding EF-hand regions were in putative exon 1. The recoverin gene was mapped to human chromosome 17 by hybridization to a panel of human-rodent hybrid DNAs. © 1992 Academic Press, Inc.
引用
收藏
页码:234 / 244
页数:11
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