EFFECT OF CARBON AND NITROGEN-METABOLISM ON NITRATE REDUCTASE-ACTIVITY OF RHODOBACTER-CAPSULATUS E1F1

被引:17
作者
DOBAO, MM [1 ]
MARTINEZLUQUE, M [1 ]
MORENOVIVIAN, C [1 ]
CASTILLO, F [1 ]
机构
[1] UNIV CORDOBA,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOLEC,E-14071 CORDOBA,SPAIN
关键词
CARBON-NITROGEN BALANCE; NITRATE REDUCTASE; RHODOBACTER CAPSULATUS;
D O I
10.1139/m94-102
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The phototrophic bacterium Rhodobacter capsulatus E1F1 possesses an assimilatory, inducible nitrate reductase that is regulated by carbon and nitrogen metabolism. Nitrate reductase activity was detected in cells cultured with amino acids and nitrate as simultaneous nitrogen source but it required an additional carbon source such as D,L-malate. A significant rise in nitrate reductase activity was observed in media with increasing nitrate concentrations up to 10 mM KNO3, although higher nitrate concentrations had an inhibitory effect. Growth yield, generation time, and nitrate reductase activity were also dependent on the concentration of D,L-malate in cells growing with 10 mM nitrate. In carbon-starved cells, nitrate reductase activity dropped even in the presence of nitrate. The intracellular concentration of keto acids such as oxaloacetate or 2-oxoglutarate fluctuated widely depending on the presence of nitrogen and carbon sources in the culture medium. The increase in the intracellular concentration of oxaloacetate or 2-oxoglutarate in R. capsulatus E1F1 correlated well with a rise in nitrate reductase activity. These results suggest that the intracellular carbon-nitrogen balance regulates nitrate uptake in R. capsulatus E1F1, thus affecting the expression of nitrate reductase.
引用
收藏
页码:645 / 650
页数:6
相关论文
共 33 条
  • [1] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [2] BURLINA A, 1985, METHOD ENZYMAT AN, V7, P20
  • [3] A MEMBRANE-BOUND DISSIMILATORY NITRATE REDUCTASE FROM RHODOBACTER-SPHAEROIDES F-SP DENITRIFICANS
    BYRNE, MD
    NICHOLAS, DJD
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 915 (01) : 120 - 124
  • [4] NITRITE UPTAKE SYSTEM IN PHOTOSYNTHETIC BACTERIUM RHODOPSEUDOMONAS-CAPSULATA E1F1
    CABALLERO, FJ
    MORENOVIVIAN, C
    CASTILLO, F
    CARDENAS, J
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 848 (01) : 16 - 23
  • [5] CHARACTERIZATION OF UREASE FROM THE PHOTOTROPHIC BACTERIUM RHODOBACTER-CAPSULATUS E1F1
    DOBAO, MD
    CASTILLO, F
    PINEDA, M
    [J]. CURRENT MICROBIOLOGY, 1993, 27 (02) : 119 - 123
  • [6] NITRATE REDUCTASE-ACTIVITY IN SPHEROPLASTS FROM RHODOBACTER-CAPSULATUS E1F1 REQUIRES A PERIPLASMIC PROTEIN
    DOBAO, MM
    MARTINEZLUQUE, M
    CASTILLO, F
    [J]. ARCHIVES OF MICROBIOLOGY, 1993, 160 (06) : 471 - 476
  • [7] ANAEROBIC RESPIRATION IN THE RHODOSPIRILLACEAE - CHARACTERIZATION OF PATHWAYS AND EVALUATION OF ROLES IN REDOX BALANCING DURING PHOTOSYNTHESIS
    FERGUSON, SJ
    JACKSON, JB
    MCEWAN, AG
    [J]. FEMS MICROBIOLOGY LETTERS, 1987, 46 (02) : 117 - 143
  • [8] Guder WG, 1983, METHOD ENZYMAT AN, VII, P2
  • [9] DEREPRESSION OF NITROGENASE BY ADDITION OF MALATE TO CULTURES OF RHODOSPIRILLUM-RUBRUM GROWN WITH GLUTAMATE AS THE CARBON AND NITROGEN-SOURCE
    HOOVER, TR
    LUDDEN, PW
    [J]. JOURNAL OF BACTERIOLOGY, 1984, 159 (01) : 400 - 403
  • [10] MOLECULAR AND KINETIC CHARACTERIZATION OF GLUTAMATE SYNTHASE FROM THE PHOTOTROPHIC BACTERIUM RHODOBACTER-CAPSULATUS E1F1
    IGENO, MI
    CABALLERO, FJ
    CASTILLO, F
    [J]. JOURNAL OF GENERAL MICROBIOLOGY, 1993, 139 : 2921 - 2929