CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF PHENOL HYDROXYLASE FROM TRICHOSPORON-CUTANEUM

Recombinant phenol hydroxylase from the soil yeast Trichosporon cutaneum has been crystallized with PEG 4000 as precipitant. The crystals are monoclinic, space group P21 with cell dimensions a = 101.8 Å, b = 153.0 Å, c = 116.0 Å and β = 114.8°. The crystal asymmetric unit most likely contains two dimers of phenol hydroxylase corresponding to a packing density in the crystals of 2.54 Å3/Da. The self-rotation function is consistent with the packing of two dimers in the asymmetric unit. The observed diffraction pattern extends beyond 2.8 Å resolution and the crystals are well suited for structural analysis by X-ray diffraction methods.