DETECTION OF BETA-CELL-SPECIFIC DNA-DAMAGE IN STREPTOZOTOCIN-TREATED RATS BY IN-SITU NICK TRANSLATION WITH IMMUNOSTAINING OF ALPHA-CELLS

被引：8

作者：

SHIBATA, S

ASANUMA, Y

KOYAMA, K

SAITO, K

机构：

[1] AKITA UNIV, SCH MED, DEPT SURG, AKITA 010, JAPAN

[2] AKITA UNIV, SCH MED, DEPT PATHOL, AKITA 010, JAPAN

来源：

PANCREAS | 1995年 / 10卷 / 04期

关键词：

IN SITU NICK TRANSLATION; AUTORADIOGRAPHY; DNA DAMAGE; STREPTOZOTOCIN; DNA POLYMERASE I;

D O I：

10.1097/00006676-199505000-00006

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

In situ nick translation with immunostaining of a cells could demonstrate the specific localization of streptozotocin-(STZ) induced DNA damage in beta cells using in vivo materials. The extent of DNA damage was determined through autoradiography by counting the number of grains in the nucleus of the alpha, beta, and exocrine cells of the rat pancreas. Subsequently, in situ nick translation with immunostaining of alpha cells was carried out in a pancreas pretreated with STZ. The number of grains observed in the beta cells of the STZ-treated groups was significantly higher than that in the control group. DNA damage of pancreatic beta cells due to STZ could be detected visually using in situ nick translation with immunostaining of alpha cells. Moreover, it was also possible to compare the DNA damage in the individual cells of the pancreas.

引用

页码：354 / 359

页数：6

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