CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES ON THE APO FORM OF OROTATE PHOSPHORIBOSYLTRANSFERASE FROM ESCHERICHIA-COLI

被引:8
作者
AGHAJARI, N
JENSEN, KF
GAJHEDE, M
机构
[1] UNIV COPENHAGEN,HC ORSTED INST,CTR CRYSTALLOG STUDIES,INST CHEM,DK-2100 COPENHAGEN 0,DENMARK
[2] UNIV COPENHAGEN,CTR ENZYME RES,INST MOLEC BIOL,DK-1123 COPENHAGEN K,DENMARK
关键词
OPRTASE; NUCLEOTIDE SYNTHESIS; CRYSTALLIZATION; X-RAY DIFFRACTION;
D O I
10.1006/jmbi.1994.1503
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Three different crystal forms of the apoenzyme orotate phosphoribosyltransferase, with M(r)= 23,552 from Escherichia coli have been grown. The crystals, which are all suitable for X-ray diffraction analysis, have been grown by the hanging drop vapour diffusion method. The first form crystallizes in the orthorhombic space group P2(1)2(1)2, with cell dimensions: a = 136.34 Angstrom, b= 75.98 Angstrom and c = 40.32 a; the second form in the monoclinic space group P2, with unit cell dimensions: a = 101.61 Angstrom, b = 40.49 Angstrom, c = 79.05 Angstrom and beta = 87.33 degrees; and the third form in P2(1)2(1)2(1), the cell dimensions being a = 70.27 Angstrom, b = 103.53 Angstrom, c = 53.83 Angstrom.
引用
收藏
页码:292 / 294
页数:3
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