MORPHOLOGICAL DETECTION OF X-CHROMOSOMES AND Y-CHROMOSOMES IN SMEARS AND PARAFFIN-EMBEDDED TISSUES USING A NONISOTOPIC IN-SITU HYBRIDIZATION TECHNIQUE (NISH)

被引：4

作者：

BETZ, P ^{[1
]}

BARETTON, G ^{[1
]}

TUBEL, J ^{[1
]}

NERLICH, A ^{[1
]}

EISENMENGER, W ^{[1
]}

机构：

[1] UNIV MUNICH,DEPT PATHOL,D-80337 MUNICH,GERMANY

来源：

INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 1994年 / 107卷 / 02期

关键词：

IN-SITU HYBRIDIZATION; SEX DETERMINATION;

D O I：

10.1007/BF01225497

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Pharyngeal smears and paraffin-embedded tissue specimens (skeletal muscle, kidney) obtained from 10 male and 10 female individuals were evaluated using non-isotopic in situ hybridization (NISH) with commercial X- and Y-specific biotinylated probes which recognize the pericentrometric regions DXZ1 and DYZ1/DYZ3 of the X- and Y-chromosome, respectively. The results provide evidence that the morphological sex determination of a single cell can be performed by critical application of this staining method leading to one nuclear signal in ''male'' cells using the Y-specific probe whereas ''female'' cells are negative. In situ hybridization of ''female'' tissues with an X-specific probe results regularly in 2 signals whereas ''male'' cells show only one spot in the nucleus.

引用

页码：108 / 110

页数：3

共 13 条

[1] SEX DETERMINATION OF FORENSIC SAMPLES BY DUAL PCR AMPLIFICATION OF AN X-Y HOMOLOGOUS GENE [J].