ORGANIC NITRATES, THIONITRATES, PEROXYNITRITES, AND NITRIC-OXIDE - A MOLECULAR-ORBITAL STUDY OF THE RXNO(2)REVERSIBLE-ARROW-RXONO (X=O, S) REARRANGEMENT, A REACTION OF POTENTIAL BIOLOGICAL SIGNIFICANCE

The rearrangement of organic thionitrate to sulfenyl nitrite potentially mediates the release of nitric oxide from organic nitrates, such as nitroglycerin, in the presence of thiol. The biological activity of these nitrovasodilators is proposed to result from release of nitric oxide in vivo. The thionitrate rearrangement bears analogy to the rearrangement of peroxynitrous acid to nitric acid, which has been proposed to mediate the biological toxicity of nitric oxide and superoxide. In this paper, the two concerted rearrangement processes and competing homolytic reactions are explored using molecular orbital calculations at levels up to MP4SDQ/6-31G*//MP2/6-31G*. Examination of structure and energy for all conformers and isomers of RSONO(2) (R = H, Me), models for organic thionitrates and their isomers, demonstrates that structures corresponding to thionitrates and sulfenyl nitrates are of similar energy. Free energies of reaction for homolysis of these compounds are low (Delta G(0) < 19 kcal/mol), whereas the barrier for concerted rearrangement is large (Delta G(not equal)(aq.) = 56 kcal/mol). The larger barrier for concerted rearrangement of peroxynitrous acid to nitric acid (Delta G(not equal) = (aq.) = 60 kcal/mol) again compares unfavourably with homolysis (Delta G(0) < 11 kcal/mol for homolysis to NO2 or (NO)-N-.). The transition state structures, confirmed by normal mode and intrinsic reaction coordinate analysis, indicate that considerable structural reorganization is required for concerted rearrangement of the ground state species. These results suggest that concerted rearrangement is not likely to be a viable step in either biological process. However, rearrangement via homolysis and radical recombination may provide an energetically accessible pathway for peroxynitrous acid rearrangement to nitric acid and rearrangement of thionitrate to sulfenyl nitrite. In this case, NO2 will be a primary product of both reactions.