RAPID DETECTION OF LEGIONELLA SPECIES IN BRONCHOALVEOLAR LAVAGE FLUIDS WITH THE ENVIROAMP LEGIONELLA PCR AMPLIFICATION AND DETECTION KIT

被引：56

作者：

KESSLER, HH ^{[1
]}

REINTHALER, FF ^{[1
]}

PSCHAID, A ^{[1
]}

PIERER, K ^{[1
]}

KLEINHAPPL, B ^{[1
]}

EBER, E ^{[1
]}

MARTH, E ^{[1
]}

机构：

[1] KARL FRANZENS UNIV GRAZ,DEPT PEDIAT,A-8036 GRAZ,AUSTRIA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 12期

关键词：

D O I：

10.1128/JCM.31.12.3325-3328.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A molecular assay based on a rapid DNA extraction protocol and the EnviroAmp Legionella Kits was used to detect Legionella species in bronchoalveolar fluid specimens. All Legionella strains isolated from tap water in hospitals could be detected distinctly. Both sensitivity and specificity were tested. In a prospective study, bronchoalveolar lavage fluids obtained from patients with atypical pneumonia were investigated. Three positive samples were detected with the molecular techniques and were subsequently confirmed by culture. Application of the system described may lead to safe and early diagnosis of Legionnaires' disease in patients with atypical pneumonia.

引用

页码：3325 / 3328

页数：4

共 20 条

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