HUMAN PREIMPLANTATION DEVELOPMENT INVITRO IS NOT ADVERSELY AFFECTED BY BIOPSY AT THE 8-CELL STAGE

被引：248

作者：

HARDY, K

MARTIN, KL

LEESE, HJ

WINSTON, RML

HANDYSIDE, AH

机构：

[1] Institute of Obstetrics and Gynaecology, Royal Postgraduate Medical School, Hammersmith Hospital, London W12 0NN, Du Cane Road

[2] Department of Biology, University of York

来源：

HUMAN REPRODUCTION | 1990年 / 5卷 / 06期

基金：

英国医学研究理事会;

关键词：

Human embryo metabolism; Human preimplantation embryo; Inner cell mass; Preimplanation biopsy; Trophectoderm;

D O I：

10.1093/oxfordjournals.humrep.a137173

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Normally fertilized human embryos biopsied 3 days after in-vitro fertilization (IVF) have been examined for effects on viability and development in vitro / after removal of one or two cells at the 8-cell stage (1/8 and 2/8) from each embryo. A high proportion of 7/8 and 6/8 biopsied and unmanlpulated embryos developed to the blastocyst stage between days 5 and 6 (79, 71 and 59%, respectively), and many biopsied embryos (56%) hatched from the zona pellucida in vitro. The viability of biopsied embryos which developed to the blastocyst stage was assessed by daily non-invasive measurement of the uptake of two energy substrates, glucose and pyruvate. Uptake of both substrates was generally lower in 7/8 and 6/8 biopsied embryos but only in proportion to the reduced cellular mass. The total cell number and the numbers of both trophectoderm (TE) and inner cell mass (ICM) cells in biopsied embryos at the blastocyst stage, counted by differential labelling of their nuclei, were also reduced in proportion but the ratio of ICM to TE cells was maintained in both 7/8 and 6/8 biopsied embryos. We conclude that removal of one or two cells at the 8-cell stage, while reducing the cellular mass, does not adversely affect the preimplantation development of biopsied embryos in vitro and suggest that this approach could be used for preimplantation diagnosis of genetic defects. © 1990 Oxford University Press.

引用

页码：708 / 714

页数：7

共 27 条

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