START-UP OF A THERMOPHILIC UPFLOW ANAEROBIC SLUDGE BED (UASB) REACTOR WITH MESOPHILIC GRANULAR SLUDGE

被引:74
作者
VANLIER, JB
GROLLE, KCF
STAMS, AJM
DEMACARIO, EC
LETTINGA, G
机构
[1] WAGENINGEN UNIV AGR,DEPT MICROBIOL,6703 CT WAGENINGEN,NETHERLANDS
[2] SUNY ALBANY,NEW YORK STATE DEPT HLTH,WADSWORTH CTR LABS & RES,ALBANY,NY 12210
[3] SUNY ALBANY,SCH PUBL HLTH,ALBANY,NY 12210
关键词
D O I
10.1007/BF00174217
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Fast start-up of thermophilic upflow anaerobic sludge bed (UASB) reactors was achieved at process temperatures of 46, 55 and 64-degrees-C, using mesophilic granular sludge as inoculum and fatty acid mixtures as feed. The start-up was brought about by increasing the temperature of mesophilic UASB reactors in a single step, which initially led to a sharp drop in the methane production rate. Thereafter, stable thermophilic methanogenesis was achieved within a period of 1 or 2 weeks depending on the temperature of operation. Mesophilic granules functioned initially as effective carrier material for thermophilic organisms. However, long-term operation led to disintegration of the granules, resulting in wash-out of thermophilic biomass. The temperature optima for acetotrophic methanogenic activity of the sludges cultivated at 46, 55 and 64-degrees-C, were similar, but differed significantly from the temperature optimum of the mesophilic inoculum. All the sludges examined were dominated by Methanothrix-like rods. These could be distinguished by antigenic fingerprinting into two subpopulations, one predominant at 36-degrees-C and the other predominant at 46-degrees-C and above.
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页码:130 / 135
页数:6
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