SPECIFICITY OF MUCOR-MIEHEI LIPASE ON METHYL-ESTER SUBSTRATES

被引:11
作者
AGGELIS, G [1 ]
KOMAITIS, M [1 ]
PINA, M [1 ]
GRAILLE, J [1 ]
机构
[1] AGR UNIV ATHENS,DEPT AGR BIOL & BIOTECHNOL,GR-11855 ATHENS,GREECE
关键词
LIPASE (MUCOR MIEHEI); METHYL ESTER; SPECIFICITY; SUBSTRATE;
D O I
10.3989/gya.1993.v44.i6.1058
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Fatty acid methyl esters constitute a good substrate for the characterization of lipase typospecificity. In the present work, the hydrolytic action of lipase from Mucor miehei was studied. It was demonstrated that this lipase preferentially catalyses the hydrolysis of fatty acid methyl esters with small number of double bonds. It was also found that this lipase shows a specificity in the hydrolysis of fatty acid methyl esters with short aliphatic chain.
引用
收藏
页码:331 / 334
页数:4
相关论文
共 21 条
  • [1] Aggelis G., Pina M., Ratomahenina F.L., Arnaund A., Graille J., Galzy P., Martin-Privat P., Perraud J.P., «production d'huiles riches en acide gamma linolenique par diverses souches de Phycomy-cetes», Oléagineux, 42, pp. 379-386, (1987)
  • [2] «recueil de Normes Fraņaises des Corps Gras, Graines Oléagineux et Produites Dérives», (1984)
  • [3] Chang M.-K., Abraham G., John V.T., Production of cocoa butter-like fat from interesterification of vegetable oils, J. Am. Oil Che-mists'Soc, 67, pp. 832-834, (1990)
  • [4] Galzy P., Ratomahenina R., Graille J., Pina M., Microbiologie appliquée a l'industrie des lipides, Rev. Fr. Corps Gras, 33, pp. 307-309, (1986)
  • [5] Jensen R.G., «microbial lipolytic enzymes, Lipids, 9, pp. 149-152, (1974)
  • [6] Ghoshray S., Bhattacharya D.K., Enzymatic preparation of ricinoleic acid esters of long-chain monohydric alcohols and properties of the esters, %J. Am. Oil chemists'Soc, 69, pp. 85-88, (1992)
  • [7] Hills M., Klewitt I., Mukherjee K., Enrichment of y-linolenic acid and docosahexaenoic acid by selective esterification catalyzed by lipases, %J. Am. Oil chemists'Soc, 67, pp. 561-564, (1990)
  • [8] Langholz P., Andersen P., Forskov T., Schmidtsdorff W., Application of a specificity of mucor miehei lipase to concentrate docosahexaenoic acid (dha), J. Am. Oil chemists'Soc, 66, pp. 1120-1123, (1989)
  • [9] Macrae A.R., Lipase-cǎtalyzed interesterification of oils and fats, J. Am. Oil chemists'Soc, 60, pp. 291-294, (1983)
  • [10] Muderhwa J.M., Pina M., Graille J., «probleme du dosage des acides gras en positions internes et externes des triglycerides des huiles lauriques