FLUORESCENCE LIFETIME IMAGING MICROSCOPY (FLIM) - INSTRUMENTATION AND APPLICATIONS

被引：82

作者：

WANG, XF ^{[1
]}

PERIASAMY, A ^{[1
]}

HERMAN, B ^{[1
]}

COLEMAN, DM ^{[1
]}

机构：

[1] WAYNE STATE UNIV,DEPT CHEM,DETROIT,MI 48202

来源：

CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY | 1992年 / 23卷 / 05期

关键词：

FLUORESCENCE SPECTROSCOPY; TIME-RESOLVED FLUORESCENCE SPECTROSCOPY; FLUORESCENCE MICROSCOPY; FLUORESCENCE LIFETIME IMAGING; TIME-RESOLVED FLUORESCENCE MICROSCOPY; FLUORESCENCE LIFETIME IMAGING MICROSCOPY; CONFOCAL FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; IMAGE INTENSIFIER; INSTRUMENTATION;

D O I：

10.1080/10408349208051651

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The new and novel techniques of fluorescence lifetime imaging (FLI)** and fluorescence lifetime imaging microscopy (FLIM) provide the investigator with the capacity to quantitate two-dimensional fluorescence intensity distributions and lifetimes. The concept, theory, and instrumentation of FLI and FLIM are reviewed in this paper. The implementation of FLIM instrumentation with conventional and confocal microscopic systems is discussed. These instruments permit the quantitative measurement of molecular interactions and chemical environment from samples in biological, physical, and environmental sciences. Numerous applications in the biomedical sciences for FLIM instrumentation are also discussed.

引用

页码：369 / 395

页数：27

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