THE EFFECT OF GLUCOSINOLATES (MUSTARD OIL GLYCOSIDES) AND PRODUCTS OF THEIR ENZYMATIC DEGRADATION ON THE INFECTIVITY OF TURNIP MOSAIC-VIRUS

The effect of glucosinolates sinigrin, progoitrin, epiprogroitrin, gluconapin, gluconapoleiferin, glucobrassicanapin, glucotropaeolin and gluconasturtiin without and with the glucosinolate-degrading enzyme (myrosinase, EC:3.2.3.1.), on the infectivity of turnip mosaic virus was studied. Little or no effect was observed when the intact glucosinolate (2.5 mumol cm-3) was added to the suspension of turnip mosaic virus (TuMV, isolate Ruzyne; 0.2 mg cm-3 in 0.01 M potassium phosphate buffer) at both pH 7 and pH 6. A significant decrease of virus infectivity was, however, observed when 0.25, 1.25 and 2.5 mumol cm-3 of the glucosinolate together with 0.31, 1.56 and 3.13 mg cm-3 of the myrosinase, respectively, was added to the virus suspension of both pH 7 and pH 6. The effect, which was greater at pH 6, was most intense with sinalbin and glucobrassicin substrates.