COMPLEX STRUCTURE OF THE COMMON CAROTID-ARTERY OF SHEEP

Background: Mechanical properties of blood vessels are dictated by the vessel wall structure. In many large conduit vessels the tunica media is a sheath of circular musculature and the tunica adventitia a layer of fibrous connective tissue with limited longitudinal extensibility. In contrast, the carotid artery of the sheep displays in each tunica a more complex architecture of muscle and connective tissue. Methods: Vessels collected from ewes were measured and processed for light microscopy and for transmission electron microscopy, Results: Layers of histologically different materials are found within the tunica intima, media and adventitia. (1) The tunica media is made of circumferentially arranged muscle cells markedly different at different depths, In the innermost third of the media, muscle cells are small and with irregular profiles, the cells are widely separated, and the extracellular material is abundant and composed mainly of elastic fibres. In the outermost third, muscle cells are larger and with more regular profiles, the cells are relatively close to each other and the extracellular material is sparse and consists mainly of collagen fibrils. (2) A small number of fibroblasts is found in all parts of the media amongst the preponderant muscle cells, (3) The intima contains fibroblast-like cells and longitudinally arranged muscle cells, (4) The adventitia contains a thick layer of collagen and elastic fibres; external to this, it displays a conspicuous musculature, made of large bundles of longitudinal muscle. Conclusions: The carotid artery of the sheep presents in all three coats of its wall features which are at variance from those in the better known vessels of small laboratory animals. The presence of many layers of material within the wall, the heterogeneity of the tissues found, and the occurrence of an extensive longitudinal musculature, have important effects on the mechanical properties of the vessel. (C) 1995 Wiley-Liss, Inc.