DETERMINATION OF D-AMPHETAMINE IN BIOLOGICAL SAMPLES USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER PRECOLUMN DERIVATIZATION WITH O-PHTHALDIALDEHYDE AND 3-MERCAPTOPROPIONIC ACID

被引：21

作者：

BOWYER, JF

CLAUSING, P

NEWPORT, GD

机构：

[1] Division of Neurotoxicology, National Center for Toxicological Research, Jefferson, AR 72079-9502

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 666卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00573-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An HPLC method is described for the determination of amphetamine using fluorometric detection after derivatization with o-phthaldialdehyde and 3-mercaptopropionic acid. This procedure is more sensitive (detection limit 370 fmol in microdialysate buffer standards, 1.5 pmol in extracted plasma and tissue samples) than most of the previous methods described for the determination of amphetamine with HPLC-fluorescence detection. Due to the stability of the derivative it is also suitable for autosampling after manual derivatization. Investigators currently using o-phthaldialdehyde derivatization and fluorometric detection for amino acid determination should be able to rapidly implement this method.

引用

页码：241 / 250

页数：10

共 25 条

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