INTERMEDIATE FILAMENT CDNAS FROM BHK-21-CELLS - DEMONSTRATION OF DISTINCT GENES FOR DESMIN AND VIMENTIN IN ALL VERTEBRATE CLASSES

被引：58

作者：

QUAX, W

VANDENHEUVEL, R

EGBERTS, WV

QUAXJEUKEN, Y

BLOEMENDAL, H

机构：

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES | 1984年 / 81卷 / 19期

关键词：

D O I：

10.1073/pnas.81.19.5970

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Recombinant cDNA plasmids for the intermediate filament proteins desmin and vimentin were constructed from baby hamster kidney (BHK-21) mRNA. Analysis of 4 desmin clones gave a sequence of 1574 nucleotides, which is 75% of the total mRNA length. The derived amino acid sequence for hamster desmin shows 92% overall homology with chicken desmin. The homology with hamster vimentin is highest in the .alpha.-helical middle part (74%). The 3''-noncoding region of desmin mRNA was 677 nucleotides long. With the aid of 5''- and 3''-specific probes, it was established that there is a single gene for desmin in the hamster genome. This gene expresses a single mRNA species of 2.2 kilobases. Hybridization experiments of a number of DNA with desmin and vimentin probes show that there are distinct restriction enzyme fragments carrying vimentin and desmin sequences in the genome of representatives of all vertebrate classes.

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页码：5970 / 5974

页数：5

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