HEPATIC LIPASE MESSENGER-RNA IS EXPRESSED IN RAT AND HUMAN STEROIDOGENIC ORGANS

被引：23

作者：

VERHOEVEN, AJM

JANSEN, H

机构：

[1] Department of Biochemistry, Erasmus University Rotterdam, 3000 DR Rotterdam

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1994年 / 1211卷 / 01期

关键词：

HEPATIC TRIACYLGLYCEROL LIPASE; ADRENAL; OVARY; TESTIS; CUSHINGS DISEASE; (HUMAN); (RAT);

D O I：

10.1016/0005-2760(94)90146-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat and human steroidogenic organs contain an enzyme activity that is indistinguishable from hepatic lipase present in liver. Using primers that recognize exons 5 and 8 of the rat and human HL gene, a 596-bp product was found by RT-PCR in rat liver, adrenal, ovaries and testes, but not in heart and kidney. A similar product was also observed with human hyperplastic adrenocortical tissue. Identity of this product with part of the HL cDNA was confirmed by restriction mapping and internal re-amplification. Our results indicate that the HL gene is transcribed in steroidogenic tissues that also contain HL protein.

引用

页码：121 / 124

页数：4

共 24 条

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[2]

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[3]

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