CHEMILUMINESCENCE INTENSITIES AND SPECTRA OF LUMINOL OXIDATION BY SODIUM-HYPOCHLORITE IN THE PRESENCE OF HYDROGEN-PEROXIDE

被引：41

作者：

ARNHOLD, J

MUELLER, S

ARNOLD, K

GRIMM, E

机构：

[1] Institute of Biophysics, School of Medicine, University of Leipzig

来源：

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE | 1991年 / 6卷 / 03期

关键词：

LUMINOL; SODIUM HYPOCHLORITE; HYDROGEN PEROXIDE; CHEMILUMINESCENCE INTENSITIES; CHEMILUMINESCENCE SPECTRA;

D O I：

10.1002/bio.1170060309

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydrogen peroxide amplifies the chemiluminescence in the odixation of luminol by sodium hypochlorite. A linear relationship between concentration of hydrogen peroxide and light intensity was found in the concentration range 5 x 10(-8)-7.5 x 10(-6) mol/l. At 7.5 x 10(-6) mol/l H2O2 the chemiluminescence is amplified 550-fold. The chemiluminescence spectra of these reactions have a wavelength maximum at 431 nm independent of the concentration of hydrogen peroxide. The results indicate that hydrogen peroxide is a necessary component in the chemiluminescent oxidation of the luminol by sodium hypochlorite.

引用

页码：189 / 192

页数：4

共 14 条

[1] BIOLOGICAL REACTIVITY OF HYPOCHLOROUS ACID - IMPLICATIONS FOR MICROBICIDAL MECHANISMS OF LEUKOCYTE MYELOPEROXIDASE
ALBRICH, JM
MCCARTHY, CA
HURST, JK
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (01): : 210 - 214
[2] PHAGOCYTIC ACTIVATION OF A LUMINOL-DEPENDENT CHEMILUMINESCENCE IN RABBIT ALVEOLAR AND PERITONEAL MACROPHAGES
ALLEN, RC
LOOSE, LD
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1976, 69 (01) : 245 - 252
[3] ALLEN RC, 1986, METHOD ENZYMOL, V133, P449
[4] BEERS RF, 1952, J BIOL CHEM, V195, P133
[5] CO-OXIDATION OF LUMINOL BY HYPOCHLORITE AND HYDROGEN-PEROXIDE IMPLICATIONS FOR NEUTROPHIL CHEMI-LUMINESCENCE
BRESTEL, EP
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 126 (01) : 482 - 488
[6] ROLE OF MYELOPEROXIDASE IN LUMINOL-DEPENDENT CHEMI-LUMINESCENCE OF POLYMORPHONUCLEAR LEUKOCYTES
DAHLGREN, C
STENDAHL, O
[J]. INFECTION AND IMMUNITY, 1983, 39 (02) : 736 - 741
[7] DECHATELET LR, 1982, J IMMUNOL, V129, P1589
[8] Govorova N Iu, 1987, Biokhimiia, V52, P1670
[9] CHEMI-LUMINESCENCE MECHANISM OF CYCLIC HYDRAZIDES SUCH AS LUMINOL IN AQUEOUS-SOLUTIONS
LIND, J
MERENYI, G
ERIKSEN, TE
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1983, 105 (26) : 7655 - 7661
[10] NUCLEOPHILIC-ADDITION TO DIAZAQUINONES - FORMATION AND BREAKDOWN OF TETRAHEDRAL INTERMEDIATES IN RELATION TO LUMINOL CHEMILUMINESCENCE
MERENYI, G
LIND, J
ERIKSEN, TE
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (24) : 7716 - 7726

← 1 2 →