CELL-CYCLE-SPECIFIC F-PLASMID REPLICATION - REGULATION BY CELL-SIZE CONTROL OF INITIATION

被引:34
作者
KEASLING, JD
PALSSON, BO
COOPER, S
机构
[1] UNIV MICHIGAN,SCH MED,DEPT MICROBIOL & IMMUNOL,ANN ARBOR,MI 48109
[2] UNIV MICHIGAN,DEPT CHEM ENGN,ANN ARBOR,MI 48109
关键词
D O I
10.1128/jb.173.8.2673-2680.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
F plasmid replication during the Escherichia coli division cycle was investigated by using the membrane-elution technique to produce cells labeled at different times during the division cycle and scintillation counting for quantitative analysis of radioactive plasmid DNA. The F plasmid replicated, like the minichromosome, during a restricted portion of the bacterial division cycle; i.e., F plasmid replication is cell-cycle specific. The F plasmid replicated at a different time during the division cycle than a minichromosome present in the same cell. F plasmid replication coincided with doubling in the rate of enzyme synthesis from a plasmid-encoded gene. When the cell cycle age of replication of the F plasmid was determined over a range of growth rates, the cell size at which the F plasmid replicated followed the same rules as did replication of the bacterial chromosome-initiation occurred when a constant mass per origin was achieved-except that the initiation mass per origin for the F plasmid was different from that for the chromosome origin. In contrast, the high-copy mini-R6K plasmid replicated throughout the division cycle.
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页码:2673 / 2680
页数:8
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