NITRIC-OXIDE DONOR-MEDIATED KILLING OF BIOLUMINESCENT ESCHERICHIA-COLI

被引：32

作者：

VIRTA, M

KARP, M

VUORINEN, P

机构：

[1] UNIV TURKU,DEPT BIOTECHNOL,SF-20520 TURKU,FINLAND

[2] ABO AKAD UNIV,DEPT BIOCHEM & PHARM,TURKU,FINLAND

[3] UNIV TURKU,DEPT BIOCHEM,SF-20520 TURKU,FINLAND

[4] TAMPERE UNIV HOSP,DEPT CLIN MICROBIOL,TAMPERE,FINLAND

[5] UNIV TAMPERE,DEPT BIOMED SCI,SF-33101 TAMPERE,FINLAND

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1994年 / 38卷 / 12期

关键词：

D O I：

10.1128/AAC.38.12.2775

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The antimicrobial activities of two nitric oxide-releasing compounds against Escherichia coli were investigated by using recombinant E. coli cloned with a luciferase gene from Pyrophorus plagiophthalamus. Since luciferase uses intracellular ATP to generate visible light which can be measured from living cells in real time, we wanted to compare the extent to which cell viability parallels light emission. Results from luminescence measurements and CFU counts were in good agreement, and the decrease in light emission was shown to provide a rapid and more sensitive indication of cytotoxicity.

引用

页码：2775 / 2779

页数：5

共 30 条

[1] APPARENT HYDROXYL RADICAL PRODUCTION BY PEROXYNITRITE - IMPLICATIONS FOR ENDOTHELIAL INJURY FROM NITRIC-OXIDE AND SUPEROXIDE [J].