RESISTANCE CHARACTERISTICS OF BLOOD CULTURE ISOLATES OF ENTEROBACTER-CLOACAE WITH SPECIAL REFERENCE TO BETA-LACTAMASES AND RELATION TO PRECEDING ANTIMICROBIAL THERAPY

Resistance characteristics of 53 blood culture isolates of E cloacae were examined and correlated with antimicrobial treatment preceding bacteraemia. Resistance patterns of 22 antimicrobial agents, presence of resistant mutants, and inducibility of beta-lactamase were investigated; furthermore, population analysis and investigation of beta-lactamase production of selected isolates were performed. Thirty-two isolates (60%) were resistant to cephalothin and/or cefoxitin and/or ampicillin, and 14 isolates (26%) had further resistance characteristics, 7 of the 14 being resistant to non-beta-lactam antibiotics. All ampicillin-susceptible and 76% of cefotaxime-susceptible isolates had resistant mutants in the zone of inhibition when high inoculum was used. All isolates investigated had inducible chromosomal beta-lactamases, and, in addition, two isolates had an enzyme corresponding with TEM-1. Correlation of resistance patterns and antimicrobial treatment preceding bacteraemia showed that treatment with a third-generation cephalosporin was associated with beta-lactam multiresistance. In conclusion, susceptibility testing of beta-lactam antibiotics of Enterobacter must be interpreted with caution and monotherapy with an extended-spectrum cephalosporin should be avoided unless presence of resistant mutants and inducibility of beta-lactamase can be excluded.