MANUAL SEQUENCING USING PULSED FIELD

被引：16

作者：

TURMEL, C ^{[1
]}

BRASSARD, E ^{[1
]}

NOOLANDI, J ^{[1
]}

机构：

[1] XEROX RES CTR CANADA LTD,2660 SPEAKMAN DR,MISSISSAUGA L5K 2L1,ONTARIO,CANADA

来源：

ELECTROPHORESIS | 1992年 / 13卷 / 9-10期

关键词：

D O I：

10.1002/elps.11501301126

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of pulsed fields in manual sequencing opens up the compression zone found with a DC field and extends the range of resolution from a few hundred bases to several thousand bases. The band inversion problem is overcome with the proper pulsing conditions, and the bands are sharper than for the DC field case. Accurate visual reading is possible up to about 800-900 bases. The method is compatible with automation techniques, since the band spectrum is stretched continuously during migration, and the smaller fragments are run off the gel.

引用

页码：620 / 622

页数：3

共 8 条

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