HUMAN PARATHYROID-HORMONE RELATED PROTEIN FRAGMENT-(1-34) HAD GLUCOSE-6-PHOSPHATE-DEHYDROGENASE ACTIVITY ON DISTAL CONVOLUTED TUBULES IN CYTOCHEMICAL BIOASSAY

被引:6
作者
NAKAI, M
FUKASE, M
SAKAGUCHI, K
NODA, T
FUJII, N
FUJITA, T
机构
[1] TOYO JOZO CO LTD, SANPUKU KU, OHITO, SHIZUOKA 41023, JAPAN
[2] KYOTO UNIV, DEPT PHARMACOL, SAKYO KU, KYOTO 606, JAPAN
关键词
D O I
10.1016/0006-291X(88)90662-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the present study, the action of parathyroid hormone related protein (PTHrP) on glucose-6-phosphate dehydrogenase (G6PD) activity of the distal convoluted tubules was examined utilizing cytochemical bioassay (CBA). Recently full amino acid residues of human PTHrP (hPTHrP), one of the causative agents of HHM, was identified based on the cDNA clone using BEN cells. We synthesized hPTHrP-(1-34) and examined the effect of this protein on G6PD activities on the distal convoluted tubules, and compared its bioactivity to that of human parathyroid hormone (hPTH)-(1-84). hPTHrP-(1-34) stimulated G6PD activity in a log linear fashion with equivalent activity to that of hPTH-(1-84) on a molar basis. Conclusively, we found that PTHrP act on distal convoluted tubules similar to hPTH.
引用
收藏
页码:146 / 150
页数:5
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