INTERACTIONS OF DELMOPINOL WITH CONSTITUENTS OF EXPERIMENTAL PELLICLE

The prolonged retention of an effective chemotherapeutic agent on oral surfaces and in dental plaque aids in plaque control. The objective of this study was to investigate interactions between delmopinol, a morpholinoethanol derivative, and experimental pellicle. Hydroxyapatite beads were coated with different constituents of pellicle (e.g., saliva, carbohydrates, cell-free enzymes, and bacteria). Delmopinol demonstrated a higher affinity for saliva-coated hydroxyapatite (sHA) and for experimental pellicle coated with in situ-synthesized glucans than for untreated hydroxyapatite. High-molecular-weight (MW) dextran but not low-MW dextran interfered with the adsorption of delmopinol to sHA. Delmopinol did not compete with dextran for the same binding sites on sHA, nor did it compete with saliva for the same binding sites on untreated hydroxyapatite. Delmopinol inhibited the activity of cell-free fructosyltransferase adsorbed onto sHA. In addition, synthesis of glucans by Streptococcus mutans adsorbed onto sHA was significantly reduced in the presence of delmopinol.