SANDWICH-TYPE FLOW-THROUGH FIBEROPTIC CELLS FOR OPTICAL ABSORBENCY MEASUREMENTS

The sandwich cell described by Pavon et al.1 and a similar sandwich cell, except with angled (45-degrees, confocal) single strand optical fibers and a conventional Z-type cell of 6-mm path length have been studied with respect to their performance for absorbance detection. Both sandwich cells show less susceptibility by one order of magnitude to artifact absorbance signals from RI changes than the Z-cell. The light throughput in the sandwich cells increase by an order of magnitude when an inert metallized reflector is used and this improves S/N. The overall light throughput is substantially greater for the angled entrance single strand fiber optic cell rather than the cell with the bifurcated fiber optic. Attainable limits of detection with these cells appear to be related to the pathlength for the cell dimensions studied.