PROPERTIES OF ANGIOTENSIN-II RECEPTORS IN GLIAL-CELLS FROM THE ADULT CORPUS-CALLOSUM

The existence and the properties of angiotensin II receptors in the adult bovine and human corpus callosum (CC) were investigated by using Xenopus oocytes and primary glial cell cultures. In oocytes injected with CC mRNA, angiotensin II elicited oscillatory Cl- currents due to activation of the inositol phosphate/Ca2+-receptor-channel coupling system. The receptors expressed in oocytes and in CC cultures were pharmacologically similar to the AT(1) receptor type as assayed by binding. Northern blot analysis and in situ hybridization studies in sections from CC and in glial cultures revealed that the receptors were molecularly related to the AT(1) receptor and that they were present in astrocytes. In these cells, activation of the receptors with angiotensin II increased de novo DNA synthesis, promoted the release of aldosterone, and induced c-Fos expression. These findings indicate that CC astrocytes possess functional AT(1) receptors that participate in various physiological processes.