ANALYSIS AND PURIFICATION OF MODIFIED METHOXY(POLYETHYLENE GLYCOL) COMPOUNDS OF SIMILAR MOLECULAR-MASS BY HIGHPERFORMANCE LIQUID-CHROMATOGRAPHY

被引：11

作者：

LEISTER, WH

WEANER, LE

WALKER, DG

机构：

[1] Department of Chemical Development, The R.W. Johnson Pharmaceutical Research Institute, Spring House

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 704卷 / 02期

关键词：

D O I：

10.1016/0021-9673(95)00217-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The chromatographic analysis of monomethoxy(polyethylene glycol) (MPEG) derivatives, with an average molecular mass of 5000, and two related PEG compounds is described. The derivatives, containing neutral, basic, and acidic groups, were separated by HPLC using a cyanopropyl-bonded analytical column with UV detection. Collected chromatographic bands were identified using H-1 NMR. MPEG-amine 2 was also derivatized to form the corresponding MPEG-semicarbazide 5B labeled with C-14 to allow for sample quantification. A two-step purification method was devised to increase the radiochemical purity of crude 5B from 50% to over 95%.

引用

页码：369 / 376

页数：8

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