ATP-DRIVEN CA2+/H+ ANTIPORT IN ACID VESICLES FROM DICTYOSTELIUM

被引：71

作者：

ROONEY, EK ^{[1
]}

GROSS, JD ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,OXFORD OX1 3QU,ENGLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 17期

基金：

英国惠康基金;

关键词：

VACUOLAR ATPASE; CA-ATPASE; ACIDIFICATION; DIFFERENTIATION; SLIME MOLD;

D O I：

10.1073/pnas.89.17.8025

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Amoebae of the cellular slime mold Dictyostelium discoideum possess an extensive and dynamic endomembrane system that includes many types of acidic vacuoles. A light membrane fraction from Dictyostelium, rich in vacuolar-type H+-ATPase, has been described [Padh, H., Lavasa, M. & Steck, T. L. (1989) J. Cell Biol. 108, 865-874]. Here, we show that this "acidosomal" fraction also contains a high-affinity vanadate-sensitive Ca2+ uptake activity that is stimulated by the pH gradient formed by the H+-ATPase. We attribute this Ca2+ uptake to the presence of a H+-countertransporting Ca2+-ATPase, pumping Ca2+ into an acidic compartment.

引用

页码：8025 / 8029

页数：5

共 30 条

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