GLOBAL TARGET ANALYSIS OF FLUORESCENCE DEPOLARIZATION IN MODEL MEMBRANES USING EXPONENTIAL SPLINES

被引：18

作者：

ARCIONI, A

TARRONI, R

ZANNONI, C

机构：

来源：

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS | 1993年 / 89卷 / 15期

关键词：

D O I：

10.1039/ft9938902815

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A generalization of the global target deconvolution procedure for the analysis of fluorescence depolarization data in membrane vesicles is proposed. This new procedure links data at different temperatures using exponential spline interpolating functions and is suitable for systems that present phase transitions. Here we have performed and analysed single-photon measurements for the probe 1,6-diphenylhexatriene (DPH) embedded in dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC) and distearoylphosphatidylcholine (DSPC) multilamellar vesicles, obtaining order parameter [P2] and rotational diffusion coefficient D(perpendicular-to) as a function of temperature.

引用

页码：2815 / 2822

页数：8

共 61 条

[1] FLUORESCENCE DEPOLARIZATION MEASUREMENTS ON ORIENTED MEMBRANES [J].

ADLER, M ;

TRITTON, TR .

BIOPHYSICAL JOURNAL, 1988, 53 (06) :989-1005

[2] RESOLVABILITY OF FLUORESCENCE LIFETIME DISTRIBUTIONS USING PHASE FLUOROMETRY [J].

ALCALA, JR ;

GRATTON, E ;

PRENDERGAST, FG .

BIOPHYSICAL JOURNAL, 1987, 51 (04) :587-596

[3] EFFECT OF ORIENTATIONAL ORDER ON THE DECAY OF THE FLUORESCENCE ANISOTROPY IN MEMBRANE SUSPENSIONS - EXPERIMENTAL-VERIFICATION ON UNILAMELLAR VESICLES AND LIPID ALPHA-LACTALBUMIN COMPLEXES [J].

AMELOOT, M ;

HENDRICKX, H ;

HERREMAN, W ;

POTTEL, H ;

VANCAUWELAERT, F ;

VANDERMEER, W .

BIOPHYSICAL JOURNAL, 1984, 46 (04) :525-539

[4] CAN [P4] BE OBTAINED FROM FLUORESCENCE DEPOLARIZATION IN LIQUID-CRYSTALS [J].

ARCIONI, A ;

TARRONI, R ;

ZANNONI, C .

NUOVO CIMENTO DELLA SOCIETA ITALIANA DI FISICA D-CONDENSED MATTER ATOMIC MOLECULAR AND CHEMICAL PHYSICS FLUIDS PLASMAS BIOPHYSICS, 1988, 10 (11) :1409-1426

[5] CAN (P4) BE OBTAINED FROM FLUORESCENCE DEPOLARIZATION IN NEMATICS - DISC-LIKE PROBES [J].