Typical neutral carriers, valinomycin and a bis(12-crown-4) derivative have been encapsulated in sol-gel-derived glasses to afford a novel type of ion-sensing membranes based on neutral carriers for ion-sensitive field-effect transistors (ISFETs). The neutral-carrier-based sol-gel-derived glass membranes, when incorporated in the ion sensors, showed high sensitivity, high selectivities, and fast potential response.