ANTIPEPTIDE ANTIBODIES DIFFERENTIATE BETWEEN LONG AND SHORT ISOFORMS OF THE D2 DOPAMINE RECEPTOR

被引：17

作者：

DAVID, C ^{[1
]}

EWERT, M ^{[1
]}

SEEBURG, PH ^{[1
]}

FUCHS, S ^{[1
]}

机构：

[1] UNIV HEIDELBERG,CTR MOLEC BIOL,MOLEC NEUROENDOCRINOL LAB,W-6900 HEIDELBERG,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 179卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91891-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed specific antibodies directed against two synthetic peptides corresponding to defined sequences in the D2 dopamine receptor. One peptide is from a region that is present only in the 'long' isoform of the receptor, whereas the other is from a region that is common to both. These antibodies are able to recognize the native receptor as judged by immunocytochemical staining of cells transfected with dopamine receptor DNA. One antibody was shown to be specific for the 'long' form of the receptor and reacts only with cells transfected with the 'long' DNA subtype and not with those transfected with the 'short' DNA subtype. This recognition is specific and can be inhibited by the corresponding free peptide and not by a non-relevant peptide. © 1991.

引用

页码：824 / 829

页数：6

共 13 条

[1] CLONING AND EXPRESSION OF A RAT D2 DOPAMINE RECEPTOR CDNA [J].

BUNZOW, JR ;

VANTOL, HHM ;

GRANDY, DK ;

ALBERT, P ;

SALON, J ;

CHRISTIE, MJ ;

MACHIDA, CA ;

NEVE, KA ;

CIVELLI, O .

NATURE, 1988, 336 (6201) :783-787

[2] HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].

CHEN, C ;

OKAYAMA, H .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2745-2752

[3] A 2ND MOLECULAR-FORM OF D2 DOPAMINE RECEPTOR IN RAT AND BOVINE CAUDATE-NUCLEUS [J].

CHIO, CL ;

HESS, GF ;

GRAHAM, RS ;

HUFF, RM .

NATURE, 1990, 343 (6255) :266-269

[4] THE DOPAMINE-D2 RECEPTOR - 2 MOLECULAR-FORMS GENERATED BY ALTERNATIVE SPLICING [J].

DALTOSO, R ;

SOMMER, B ;

EWERT, M ;

HERB, A ;

PRITCHETT, DB ;

BACH, A ;

SHIVERS, BD ;

SEEBURG, PH .

EMBO JOURNAL, 1989, 8 (13) :4025-4034

[5]

DAVID C, 1990, SOC NEUR ABSTR, V16, P205

[6] SUBUNIT SELECTIVITY AND EPITOPE CHARACTERIZATION OF MABS DIRECTED AGAINST THE GABA-A BENZODIAZEPINE RECEPTOR [J].

EWERT, M ;

SHIVERS, BD ;

LUDDENS, H ;

MOHLER, H ;

SEEBURG, PH .

JOURNAL OF CELL BIOLOGY, 1990, 110 (06) :2043-2048

[7] CLONING OF THE CDNA AND GENE FOR A HUMAN D2-DOPAMINE RECEPTOR [J].

GRANDY, DK ;

MARCHIONNI, MA ;

MAKAM, H ;

STOFKO, RE ;

ALFANO, M ;

FROTHINGHAM, L ;

FISCHER, JB ;

BURKEHOWIE, KJ ;

BUNZOW, JR ;

SERVER, AC ;

CIVELLI, O .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (24) :9762-9766

[8] AUTOMATED SYNTHESIS OF PEPTIDES [J].

MERRIFIELD, RB .

SCIENCE, 1965, 150 (3693) :178-+

[9] MONOCLONAL ANTI-ACETYLCHOLINE-RECEPTOR ANTIBODIES DIRECTED AGAINST THE CHOLINERGIC BINDING-SITE [J].

MOCHLYROSEN, D ;

FUCHS, S .

BIOCHEMISTRY, 1981, 20 (20) :5920-5924

[10] ANTIBODIES TO SYNTHETIC PEPTIDES AS PROBES FOR THE BINDING-SITE ON THE ALPHA-SUBUNIT OF THE ACETYLCHOLINE-RECEPTOR [J].

NEUMANN, D ;

GERSHONI, JM ;

FRIDKIN, M ;

FUCHS, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (10) :3490-3493

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