CDNA CLONING AND SEQUENCING OF COMPONENT C5 OF PROTEASOMES FROM RAT HEPATOMA-CELLS

被引：58

作者：

TAMURA, T

TANAKA, K

KUMATORI, A

YAMADA, F

TSURUMI, C

FUJIWARA, T

ICHIHARA, A

TOKUNAGA, F

ARUGA, R

IWANAGA, S

机构：

[1] KYUSHU UNIV 33,FAC SCI,DEPT BIOL,FUKUOKA 812,JAPAN

[2] KYUSHU UNIV 33,GRAD SCH MED SCI,DEPT MOLEC BIOL,FUKUOKA 812,JAPAN

[3] OTSUKA PHARMACEUT CO LTD,TOKUSHIMA 77101,JAPAN

来源：

FEBS LETTERS | 1990年 / 264卷 / 01期

关键词：

cDNA cloning; Multicatalytic proteinase; Proteasome; Subunit C5;

D O I：

10.1016/0014-5793(90)80773-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteasomes are multicatalytic proteinase complexes consisting of a set of non-identical polypeptide subunits. A cDNA for component C5 of rat proteasomes was isolated by screening a Reuber H4TG hepatoma cell cDNA library using synthetic oligodeoxynucleotide probes corresponding to partial amino acid sequences of the protein. The polypeptide deduced from the open reading frame consisted of 240 amino acid residues with a calculated molecular weight of 26479. Computer analysis revealed little similarity of C5 to other proteins reported so far. The primary structure of C5 showed partial sequence homology to that of another component C3, but no regions of homology with the sequence of component C2. Thus C5 is concluded to be a new type of subunit of the proteasome complex. © 1990.

引用

页码：91 / 94

页数：4

共 7 条

[1] MOLECULAR-CLONING OF CDNA FOR PROTEASOMES (MULTICATALYTIC PROTEINASE COMPLEXES) FROM RAT-LIVER - PRIMARY STRUCTURE OF THE LARGEST COMPONENT (C2) [J].