TRANSDUCING PHAGES IN GLUTAMIC ACID-PRODUCING BACTERIA

被引：22

作者：

MOMOSE, H ^{[1
]}

MIYASHIRO, S ^{[1
]}

OBA, M ^{[1
]}

机构：

[1] AJINOMOTO CO INC, CENT RES LABS, KAWASAKI, JAPAN

来源：

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 1976年 / 22卷 / 03期

关键词：

D O I：

10.2323/jgam.22.119

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Transducing phages were sought among temperate phages harbored by coryneform and glutamic acid-producing bacteria. By using a nonlysogenic subline of Brevibacterium flavum strain 2247 as the phage indicator, 24 temperate phages were isolated from strains of coryneform and unidentified glutamic acid-producing bacteria. Phage CP-119 transduced various markers at frequencies of .apprx. 10-7, and the addition of 4 mM c[cyclic]AMP to recipient cultures stimulated the transduction. Phage CP-123 transduced the trp marker at a frequency of .apprx. 10-6 when 1.2 M MgCl2 was added to the reaction mixture. Characteristics of these transducing phages and of the lysogeny states of glutamic acid-producing bacteria were discussed.

引用

页码：119 / 129

页数：11

共 17 条

[1]

ABE S, 1972, MICROBIAL PRODUCTION, P39

[2]

ADAMS MH, 1959, BACTERIOPHAGES, P454

[3]

DEMAIN AL, 1973, GENET IND MICROORGAN, V1, P19

[4] PROPHAGE P1 AN EXTRACHROMOSOMAL REPLICATION UNIT [J].