GENE TARGETING AND GENE TRAP SCREENS USING EMBRYONIC STEM-CELLS - NEW APPROACHES TO MAMMALIAN DEVELOPMENT

被引:53
作者
JOYNER, AL
机构
[1] Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Division of Molecular and Developmental Biology, Toronto, Ontario, M5G 1X5
关键词
D O I
10.1002/bies.950131206
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mouse embryonic stem cell lines offer an attractive route for introducing rare genetic alternations into the gene pool since the cells can be pre-screened in culture and the mutations then transmitted into the germline through chimera production. Two applications of this technique that seem ideally suited for a genetic analysis of development are enhancer and gene trap screens for loci expressed during gastrulation and production of targeted mutations using homologous recombination. These approaches should greatly increase the number of mouse developmental mutants available and help to elucidate the genetic hierarchy controlling embryogenesis.
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页码:649 / 656
页数:8
相关论文
共 50 条
[1]   TRANSGENES AS PROBES FOR ACTIVE CHROMOSOMAL DOMAINS IN MOUSE DEVELOPMENT [J].
ALLEN, ND ;
CRAN, DG ;
BARTON, SC ;
HETTLE, S ;
REIK, W ;
SURANI, MA .
NATURE, 1988, 333 (6176) :852-855
[2]   TRANSCRIPT ANALYSIS OF GLP-1 AND LIN-12, HOMOLOGOUS GENES REQUIRED FOR CELL-INTERACTIONS DURING DEVELOPMENT OF C-ELEGANS [J].
AUSTIN, J ;
KIMBLE, J .
CELL, 1989, 58 (03) :565-571
[3]   P-ELEMENT-MEDIATED ENHANCER DETECTION - A VERSATILE METHOD TO STUDY DEVELOPMENT IN DROSOPHILA [J].
BELLEN, HJ ;
OKANE, CJ ;
WILSON, C ;
GROSSNIKLAUS, U ;
PEARSON, RK ;
GEHRING, WJ .
GENES & DEVELOPMENT, 1989, 3 (09) :1288-1300
[4]   SEARCHING FOR PATTERN AND MUTATION IN THE DROSOPHILA GENOME WITH A P-LACZ VECTOR [J].
BIER, E ;
VAESSIN, H ;
SHEPHERD, S ;
LEE, K ;
MCCALL, K ;
BARBEL, S ;
ACKERMAN, L ;
CARRETTO, R ;
UEMURA, T ;
GRELL, E ;
JAN, LY ;
JAN, YN .
GENES & DEVELOPMENT, 1989, 3 (09) :1273-1287
[5]  
BRADY RM, 1984, NATURE, V309, P225, DOI 10.1038/309225a0
[6]   ANALYSIS OF MAMMALIAN-CELL GENETIC-REGULATION INSITU BY USING RETROVIRUS-DERIVED PORTABLE EXONS CARRYING THE ESCHERICHIA-COLI LACZ GENE [J].
BRENNER, DG ;
CHAO, SL ;
COHEN, SN .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (14) :5517-5521
[7]   TARGETED CORRECTION OF A MAJOR HISTOCOMPATIBILITY CLASS-II E-ALPHA-GENE BY DNA MICROINJECTED INTO MOUSE EGGS [J].
BRINSTER, RL ;
BRAUN, RE ;
LO, D ;
AVARBOCK, MR ;
ORAM, F ;
PALMITER, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (18) :7087-7091
[8]   ALTERING THE GENOME BY HOMOLOGOUS RECOMBINATION [J].
CAPECCHI, MR .
SCIENCE, 1989, 244 (4910) :1288-1292
[9]   HIGH-FREQUENCY DISRUPTION OF THE N-MYC GENE IN EMBRYONIC STEM AND PRE-B-CELL LINES BY HOMOLOGOUS RECOMBINATION [J].
CHARRON, J ;
MALYNN, BA ;
ROBERTSON, EJ ;
GOFF, SP ;
ALT, FW .
MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (04) :1799-1804
[10]   REGIONALLY RESTRICTED DEVELOPMENTAL DEFECTS RESULTING FROM TARGETED DISRUPTION OF THE MOUSE HOMEOBOX GENE HOX-1.5 [J].
CHISAKA, O ;
CAPECCHI, MR .
NATURE, 1991, 350 (6318) :473-479