A RAPID METHOD FOR THE DETERMINATION OF THE DEGREE OF N-ACETYLATION OF CHITIN-CHITOSAN SAMPLES BY ACID-HYDROLYSIS AND HPLC

被引：54

作者：

NIOLA, F ^{[1
]}

BASORA, N ^{[1
]}

CHORNET, E ^{[1
]}

VIDAL, PF ^{[1
]}

机构：

[1] UNIV SHERBROOKE,FAC SCI APPL,DEPT GENIE CHIM,SHERBROOKE J1K 2R1,QUEBEC,CANADA

来源：

CARBOHYDRATE RESEARCH | 1993年 / 238卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1016/0008-6215(93)87001-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and rapid method for the quantitative determination of N-acetyl groups in chitin-chitosan samples consists of an acid hydrolysis conducted at high temperature with a mixture of sulfuric and oxalic acids. The acetic acid formed is determined by spectrophotometry at 210 nm after separation from the reaction mixture by HPLC. The method is valid for the entire range of degrees of acetylation and the results compare fairly to those of other techniques.

引用

页码：1 / 9

页数：9

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