CHANGES IN THE LEVELS OF RAT INTERLEUKIN-8/CINC AND GELATINASE IN THE EXUDATE OF CARRAGEENAN-INDUCED INFLAMMATION IN RATS

被引：48

作者：

NAKAGAWA, H ^{[1
]}

IKESUE, A ^{[1
]}

KATO, H ^{[1
]}

DEBUCHI, H ^{[1
]}

WATANABE, K ^{[1
]}

TSURUFUJI, S ^{[1
]}

NAGANAWA, M ^{[1
]}

MITAMURA, M ^{[1
]}

机构：

[1] INST CYTOSIGNAL RES INC,SHINAGAWA KU,TOKYO 140,JAPAN

来源：

JOURNAL OF PHARMACOBIO-DYNAMICS | 1992年 / 15卷 / 09期

关键词：

IL-8; RAT IL-8; CINC; NEUTROPHIL; CHEMOTAXIS; ELISA; GELATINASE; EXUDATE; INFLAMMATION;

D O I：

10.1248/bpb1978.15.461

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A sensitive enzyme-linked immunosorbent assay (ELISA) for rat interleukin 8/cytokine-induced neutrophil chemoattractant (CINC) has been established by using biotin-conjugated anti-CINC rabbit IgG. The biotin-streptavidin sandwich ELISA detected CINC at concentrations from 3 pg/ml up to 30 ng/ml. The concentration of CINC in the pouch fluid (exudate) of rat carrageenin-induced inflammation was measured by the ELISA. After a time lag of about 2 h, neutrophils steadily accumulated in the carrageenin/air-pouch until 8 h. Similarly, the CINC level of exudate increased after about a 2-h lag, and reached a maximum (134 ng/ml) at 8 h, and thereafter decreased to a negligible concentration at 24 h after carrageenin injection. In association with the rise in CINC level, the concentration of exudate 96-kDa gelatinase corresponding to neutrophil gelatinase/type IV collagenase increased with time. The results suggest that CINC contributes, at least in part, to the neutrophil migration into the inflammatory lesion of the carrageenin-induced inflammation in rats.

引用

页码：461 / 466

页数：6

共 25 条

[1] NEUTROPHIL-ACTIVATING PEPTIDE-1 INTERLEUKIN-8, A NOVEL CYTOKINE THAT ACTIVATES NEUTROPHILS [J].