PRESERVATION OF MESSENGER-RNA DURING INSITU HYBRIDIZATION IN THE COCHLEA

被引：29

作者：

RYAN, AF

WATTS, AG

SIMMONS, DM

机构：

[1] VET ADM MED CTR, SAN DIEGO, CA 92161 USA

[2] SALK INST BIOL STUDIES, NEURAL SYST LAB, LA JOLLA, CA 92037 USA

[3] UNIV CALIF SAN DIEGO, SCH MED, DEPT SURG OTOLARYNGOL, LA JOLLA, CA 92093 USA

[4] UNIV SO CALIF, DEPT NEUROSCI, LOS ANGELES, CA 90089 USA

[5] UNIV CALIF SAN DIEGO, SCH MED, DEPT NEUROSCI, LA JOLLA, CA 92093 USA

来源：

HEARING RESEARCH | 1991年 / 56卷 / 1-2期

关键词：

INNER EAR; GENE EXPRESSION; MESSENGER-RNA; HYBRIDIZATION; INSITU;

D O I：

10.1016/0378-5955(91)90164-5

中图分类号：

R36 [病理学]; R76 [耳鼻咽喉科学];

学科分类号：

100104 ; 100213 ;

摘要：

Specific nucleic acid sequences can be identified within cells using in situ hybridization. Hybridization for mRNA can document the distribution and amount of specific gene transcripts. Decalcification protocols used for immunohistochemistry in the cochlea were evaluated for use with in situ mRNA hybridization. No loss of mRNA was detected following the use of decalcification solutions at 4-degrees-C when paraformaldehyde was added to the EDTA solution. The primary determinant of mRNA preservation was paraformaldehyde.

引用

页码：148 / 152

页数：5

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