IMMUNOLOGICAL DETECTION OF GLYCOPROTEINS ON BLOTS BASED ON LABELING WITH DIGOXIGENIN

被引:5
作者
HASELBECK, A
HOSEL, W
机构
[1] Department of Biochemistry, R and D Biotechnology, Penzberg/OBB, D-82377, Boehringer Mannheim GmbH
关键词
GLYCOPROTEINS; DIGOXIGENIN; IMMUNOLOGICAL DETECTION;
D O I
10.1007/BF02788054
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the following, we describe the application of the DIG/anti-DIG system for the (structural) analysis of glycoproteins on blots. Special emphasis is being placed on the variety of the different DIG applications in order to obtain structural information concerning the glycoprotein carbohydrate chains. Prominent among them is the use of lectins with well-known specificities for carbohydrate structures.
引用
收藏
页码:207 / 219
页数:13
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