ANALYSIS OF ANANDAMIDE, AN ENDOGENOUS CANNABINOID SUBSTANCE, AND OF OTHER NATURAL N-ACYLETHANOLAMINES

被引：64

作者：

FONTANA, A

DIMARZO, V

CADAS, H

PIOMELLI, D

机构：

[1] CNR,IST CHIM MOLEC INTERESSE BIOL,I-80072 ARCO,ITALY

[2] INSERM,U109,F-75014 PARIS,FRANCE

来源：

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1995年 / 53卷 / 04期

关键词：

D O I：

10.1016/0952-3278(95)90130-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent reports have suggested that N-arachidonoylethanolamine (anandamide) acts as an endogenous ligand for cannabinoid receptors in mammalian brain. Here we describe methods for the extraction, purification and analysis of anandamide and related N-fatty acyl-ethanolamines (NAEs). Liquid-phase extraction, silica gel G column chromatography and thin-layer chromatography (TLC) were employed for sample fractionation. Three analytical high-performance liquid chromatography (HPLC) methods for purification of NAEs were developed. Finally, analyses of NAEs by gas chromatography/mass spectrometry (GC/MS) are described. The applications of these analytical methods to the identification of anandamide and related NAEs in cell cultures as well as of artifacts in biosynthetic studies are described.

引用

页码：301 / 308

页数：8

共 19 条

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