VARIATIONS OF SPECIFIC MESSENGER-RNA AND POLYPEPTIDE CONTENTS OF RAT-LIVER D-BETA-HYDROXYBUTYRATE DEHYDROGENASE DURING AN EXPERIMENTAL DIABETES-MELLITUS

被引：3

作者：

BAILLY, A ^{[1
]}

LONE, YC ^{[1
]}

LATRUFFE, N ^{[1
]}

机构：

[1] UNIV FRANCHE COMTE,BIOCHIM & BIOL MOLEC LAB,CNRS,UA 531,F-25030 BESANCON,FRANCE

来源：

BIOCHIMIE | 1990年 / 72卷 / 05期

关键词：

d-β-hydroxybutyrate dehydrogenase; diabetes; insulin action; mRNA expression;

D O I：

10.1016/0300-9084(90)90031-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of the rat liver d-β-hydroxybutyrate dehydrogenase (BDH) gene was investigated at different levels: the level of its specific mRNA, the protein content and the enzymatic activity. By using a cDNA probe, we found that the BDH mRNA was about 2kb and we report here that the decrease of BDH activity in diabetic rats is due to a reduction in the content of the enzyme, which is proportional to a diminution in the amount of the BDH mRNA. We also show that insulin is able to reverse this diabetes effect by restoring the level of BDH mRNA, the BDH content and thus its activity. This result indicates that in vivo the control of the expression of the BDH gene by insulin is mainly transcriptional and/or post-transcriptional (mRNA stability). © 1990.

引用

页码：351 / 354

页数：4

共 21 条

[1]

AUFFRAY C, 1980, EUR J BIOCHEM, V107, P303

[2] MODIFIED OSCILLATION BEHAVIOR AND DECREASED D-3-HYDROXYBUTYRATE DEHYDROGENASE-ACTIVITY IN DIABETIC RAT-LIVER MITOCHONDRIA [J].

BRIGNONE, JA ;

DEBRIGNONE, CMC ;

RODRIGUEZ, RR ;

BADANO, BN ;

STOPPANI, AOM .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1982, 214 (02) :581-588

[3]

BURNETTE WN, 1981, ANAL BIOCHEM, V112, P195, DOI 10.1016/0003-2697(81)90281-5

[4]

COQUARD C, 1982, BIOL CELL, V44, P189

[5]

COQUARD C, 1987, BIOL CELL, V181, P383

[6] INCREASED EXPRESSION OF THE N-MYC GENE DURING NORMAL AND NEOPLASTIC RAT-LIVER GROWTH [J].

CORRAL, M ;

PARIS, B ;

GUGUENGUILLOUZO, C ;

CORCOS, D ;

KRUH, J ;

DEFER, N .

EXPERIMENTAL CELL RESEARCH, 1988, 174 (01) :107-115

[7]

ELKEBBAJ MS, 1981, BIOCHEM BIOPH RES CO, V193, P265

[8] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[9]

FLEISCHER S, 1974, MEMBRANE PROTEINS TR, P125

[10] METABOLIC CONTROL OF THE EXPRESSION OF MITOCHONDRIAL D-BETA-HYDROXYBUTYRATE DEHYDROGENASE, A KETONE-BODY CONVERTING ENZYME [J].

KANTE, A ;

CHERKAOUIMALKI, M ;

COQUARD, C ;

LATRUFFE, N .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1033 (03) :291-297

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