SYNTHESIS OF FUNCTIONAL GABA(A) RECEPTORS IN STABLE INSECT-CELL LINES

被引：35

作者：

JOYCE, KA ^{[1
]}

ATKINSON, AE ^{[1
]}

BERMUDEZ, I ^{[1
]}

BEADLE, DJ ^{[1
]}

KING, LA ^{[1
]}

机构：

[1] OXFORD BROOKES UNIV,SCH BIOL & MOLEC SCI,OXFORD OX3 0BP,ENGLAND

来源：

FEBS LETTERS | 1993年 / 335卷 / 01期

基金：

英国惠康基金;

关键词：

INSECT CELLS (SF9); STABLE EXPRESSION; GABA(A) RECEPTOR; BETA-1; SUBUNIT; ELECTROPHYSIOLOGY;

D O I：

10.1016/0014-5793(93)80439-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have synthesised the beta 1-subunit of the bovine GABA, receptor in stable, continuous insect (Spodoptera frugiperda) cell lines. A cDNA was integrated randomly into the insect cell genome under control of a baculovirus immediate early (IE-1) gene promoter. Transformed cells were obtained by co-transfection of the insect cells with pIEK1.GR beta 1, encoding the beta 1 subunit cDNA, and pIEK1.neo, encoding the neomycin resistance gene. G-418-resistant clones were selected and expanded into continuous cell lines synthesising functional, GABA-gated, homo-oligomeric chloride channels. These cell lines had significant advantages over the transient baculovirus expression system for the characterisation of receptors using electrophysiological recording techniques.

引用

页码：61 / 64

页数：4

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