INDUCTION OF DNA-POLYMERASE IN POLYOMA VIRUS-INFECTED MOUSE CELLS REQUIRES TRANSCRIPTION AND TRANSLATION

The induction of DNA polymerase activity and of DNA synthesis in polyoma virus-infected mouse kidney cells is inhibited by actinomycin D and cycloheximide, even in cells that are already T[tumor]-antigen positive. This indicates that the induction of the DNA-synthesizing apparatus requires transcription and translation. The increase in cellular DNA polymerase activity and in the DNA synthesizing capacity follows the appearance of T-antigen only after a characteristic lag period of several hours.